{"id":218,"date":"2024-10-28T16:03:26","date_gmt":"2024-10-28T15:03:26","guid":{"rendered":"http:\/\/wwww.icp.universite-paris-saclay.fr\/cpsysbio\/?page_id=218"},"modified":"2024-11-08T11:27:26","modified_gmt":"2024-11-08T10:27:26","slug":"fluorescent-proteins","status":"publish","type":"page","link":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/en\/scientific-axes\/fluorescent-proteins\/","title":{"rendered":"Fluorescent Proteins and Biosensors for Imaging"},"content":{"rendered":"

Fluorescent Proteins and Biosensors for Imaging<\/h3>\n
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Yasmina Bousmah (Assistant Engineer), Marie Erard (Professor), Oliver N\u00fcsse (Professor), H\u00e9l\u00e8ne Pasquier (Associate Professor, HDR), Th\u00e9o Beguin (PhD student 2022\u2013), Dounia Zamiati (PhD student 2021\u2013)
\nAlumni: Fabienne Merola (Senior Researcher, retired in 2020), Hana Illichova Valenta (PhD student 2017\u20132020), Mouna Abdesselem (Postdoctoral fellow 2020\u20132022)<\/p>\n<\/div>\n<\/div><\/div><\/div>

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A detailed understanding of physiological functions requires real-time insights into cellular organization and communication. Fluorescence imaging is a key tool to achieve this, particularly through the use of probes such as fluorescent proteins from the GFP family. These proteins have the advantage of being genetically encoded and fully biocompatible. We contribute to the development of new fluorescent proteins, primarily cyan and yellow variants, which we use to study various biological phenomena, notably through F\u00f6rster Resonance Energy Transfer (FRET) and its detection via fluorescence lifetime imaging (FLIM).<\/p>\n

Our projects require an environment enabling experiments that range from biochemistry or molecular and cellular biology to in vitro photophysical studies and fluorescence imaging, available through the SpICy technical platform<\/a>.<\/p>\n<\/div>\n<\/div><\/div>

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Structure of fluorescent proteins and confocal images. Fluorescent proteins targeted to the membrane: Aquamarine on the left ([Erard 2013<\/a>), Addgene: pAquaN1, Plasmid #42888, pProEX-Aqua, Plasmid #42889) and tdLanYFP on the right [Bousmah 2021<\/a>].<\/em><\/p>\n<\/div>\n<\/div><\/div>

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Our activities are divided into three main areas:<\/p>\n