{"id":278,"date":"2024-10-28T17:16:31","date_gmt":"2024-10-28T16:16:31","guid":{"rendered":"http:\/\/wwww.icp.universite-paris-saclay.fr\/cpsysbio\/?page_id=278"},"modified":"2024-10-28T17:16:31","modified_gmt":"2024-10-28T16:16:31","slug":"radical-oxidation-of-biomolecules","status":"publish","type":"page","link":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/en\/scientific-axes\/oxidative-stress\/radical-oxidation-of-biomolecules\/","title":{"rendered":"Radical Oxidation of Biomolecules"},"content":{"rendered":"

Radical Oxidation of Biomolecules<\/h3>\n
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Chantal Hou\u00e9 Levin (Professor Emerita)<\/p>\n<\/div>\n<\/div><\/div><\/div>

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Methionine is one of the amino acids most susceptible to oxidation and therefore a target of reactive oxygen species (ROS) in proteins. The first step has been known for a long time [Archirel2016<\/a>, Neschchadin2014<\/a>] and leads to free radicals with 2 centers and 3 electrons characterized in pulse radiolysis and quantum chemistry, but the products formed other than methionine sulfoxide at the end of the process are not well understood. We have thus undertaken a thorough study of their characterization in model molecules containing the thioether function: small peptides in which methionine is linked to various residues and at various positions in the polypeptide chain (N-terminal, C-terminal, mid-chain) ) [Archirel2019<\/a>, Gregori2017<\/a>]. Characterization was performed by mass spectrometry coupled with infrared spectroscopy (CLIO). Quantum chemistry calculations allowed us to propose mechanisms and geometries for these products [Berg\u00e8s2020<\/a>]. We have thus identified previously unknown products, including double bonds, cycles, etc. [Jiang2022<\/a>, Jiang2023<\/a>]. More recently, we turned to the oxidation of DNA bases, both purines and pyrimidines [Ma2018<\/a>], and showed that HO\u00b0 radicals led only to hydroxylation [Jiang2023<\/a>]. Finally, regarding protein oxidation, we use gamma radiolysis to test the stability properties of various proteins (ancestral and resurrected proteins) with respect to heat and ionizing radiation [Halgand2020<\/a>].<\/p>\n<\/div>\n<\/div><\/div>

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Some examples of free radicals in the Gly Met peptide possessing a 2-center and 3-electron bond. 1 SN bond; 2 SO bond; 3 SS bond in the dipeptide MetMet.<\/em><\/p>\n<\/div>\n<\/div><\/div>

Collaborations<\/h3>\n
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Pierre Archirel and Carine Clavaguera (TheoSim, ICP, Orsay), Debora Scuderi (CAPRI, ICP, Orsay), Pedro de Oliveira (TEMiC, ICP, Orsay), Krzysztof Bobrowski and Tomasz Szreder (ICHTJ, Warsaw, Poland).<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

Chantal Hou\u00e9 Levin (Professor Emerita) Methionine is one of the amino acids most susceptible to oxidation and therefore a target of reactive oxygen species (ROS) in proteins. The first step has been known for a long time [Archirel2016, Neschchadin2014] and leads to free radicals with 2 centers and 3 electrons characterized in pulse radiolysis and… Lire la suite »Radical Oxidation of Biomolecules<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":255,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"off","neve_meta_content_width":100,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","footnotes":""},"class_list":["post-278","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/pages\/278","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/comments?post=278"}],"version-history":[{"count":1,"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/pages\/278\/revisions"}],"predecessor-version":[{"id":279,"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/pages\/278\/revisions\/279"}],"up":[{"embeddable":true,"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/pages\/255"}],"wp:attachment":[{"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/media?parent=278"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}