{"id":305,"date":"2024-10-30T18:30:24","date_gmt":"2024-10-30T17:30:24","guid":{"rendered":"http:\/\/wwww.icp.universite-paris-saclay.fr\/cpsysbio\/?page_id=305"},"modified":"2024-11-19T16:45:01","modified_gmt":"2024-11-19T15:45:01","slug":"spicy-technical-platform","status":"publish","type":"page","link":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/en\/spicy-technical-platform\/","title":{"rendered":"SpICy Technical Platform"},"content":{"rendered":"

SpICy Technical Platform <\/h3>\n
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SpICy (Sp<\/u>ectromicroscopy, I<\/u>maging et Cyt<\/u>ometry) is a micro-spectroscopy platform located on the second floor of building 350. It is situated near the L2 facility, which allows for cell culture, as well as the biochemistry and molecular biology laboratories of the CPSysBio group. Various spectroscopic measurements can be performed here, such as absorption, emission, or excitation spectra, fluorescence intensity, fluorescence lifetime, fluorescence anisotropy, and luminescence measurements.<\/p>\n

Measurements can be carried out in solution\u2014using cuvettes or droplets\u2014or on adherent or non-adherent cells. Stationary and time-resolved microscopy experiments allow for localized information in 2D\/3D, while analysis of individuals within populations is possible through cytometry.<\/p>\n

This platform is accessible to all ICP members, as well as external users, typically on a collaborative basis or through support for maintenance and operational costs.<\/p>\n

SpICy is part of the CyPSy group of cytometry platforms at Paris-Saclay and the Paris-Saclay Cellular Imaging Network (RIC<\/a>).<\/p>\n

Since 2012, the CPSysBio group and the SpICy platform have co-organized an annual CNRS Formation Entreprises training session in cytometry.<\/a><\/p>\n<\/div>\n<\/div><\/div>

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Overview of the measurements available on the SpICy technical platform<\/em>.<\/p>\n<\/div>\n<\/div><\/div>

Spectrophotometers : plate reader, LOLITA, BEAM<\/h3>\n
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The SAFAS plate reader<\/strong> is a commercial instrument that allows measurements of absorbance, fluorescence, or luminescence in both kinetic and spectral modes. This instrument is equipped with temperature regulation and sample agitation functions, and also offers the possibility to work in a spectroscopic cuvette.<\/p>\n<\/div>\n<\/div><\/div>

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SAFAS plate reader and an example of kinetic data.<\/em><\/p>\n<\/div>\n<\/div><\/div>

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LOLITA<\/strong> is a setup designed to acquire fluorescence emission decay data with high statistical and temporal quality in the 0.1-100 ns range, on solutions or powder\/solid samples. The excitation source is a white laser (440-650 nm) and UV LEDs. Polarizers in the optical path allow the recording of fluorescence anisotropy decay, providing insights into the hydrodynamic volume of the emissive species or information about the oligomeric nature of a fluorescent protein.<\/p>\n<\/div>\n<\/div><\/div>

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LOLITA setup and fluorescence emission decay of yellow fluorescent proteins, with the IRF shown in gray<\/em>.<\/p>\n<\/div>\n<\/div><\/div>

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BEAM<\/strong> is a setup that enables controlled irradiations (in terms of time and power), the triggering, and analysis of photoreactions. It facilitates simultaneous acquisition of absorption and fluorescence emission spectra (210-700 nm) and kinetic monitoring. Currently used to study the photoreactions of fluorescent proteins, it is open to other areas of research.<\/p>\n<\/div>\n<\/div><\/div>

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BEAM setup and examples of kinetic tracking of absorption and fluorescence emission spectra of a yellow fluorescent protein under irradiation.<\/em><\/p>\n<\/div>\n<\/div><\/div>

Contact<\/h3>\n
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H\u00e9l\u00e8ne Pasquier<\/a><\/p>\n<\/div>\n<\/div><\/div><\/div>

Microscopes: FloRa and BiFluor <\/h3>\n
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FloRa<\/strong> is a wide-field microscope that allows dynamic multicolor or ratiometric imaging.<\/p>\n