{"id":319,"date":"2024-10-31T14:05:39","date_gmt":"2024-10-31T13:05:39","guid":{"rendered":"http:\/\/wwww.icp.universite-paris-saclay.fr\/cpsysbio\/?page_id=319"},"modified":"2024-11-19T16:57:07","modified_gmt":"2024-11-19T15:57:07","slug":"technical-equipment","status":"publish","type":"page","link":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/en\/technical-equipment\/","title":{"rendered":"Technical Equipment"},"content":{"rendered":"<div id=\"pl-319\"  class=\"panel-layout\" ><div id=\"pg-319-0\"  class=\"panel-grid panel-no-style\" ><div id=\"pgc-319-0-0\"  class=\"panel-grid-cell\" ><div id=\"panel-319-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child\" data-index=\"0\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t><h3 class=\"widget-title\">Technical Equipment of the CPSysBio Group<\/h3>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<p>The CPSysBio group has an extensive range of instruments that support not only biochemical, molecular, and cellular biology experiments but also the detailed characterization of nanoparticles. Accessible to group members and the ICP, the equipment is also available to external users under specific conditions.<\/p>\n<\/div>\n<\/div><\/div><div id=\"panel-319-0-0-1\" class=\"so-panel widget widget_sow-image\" data-index=\"1\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-image so-widget-sow-image-default-c67d20f9f743-319\"\n\t\t\t\n\t\t>\n<div class=\"sow-image-container\">\n\t\t<img \n\tsrc=\"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-content\/uploads\/sites\/4\/2024\/10\/PI-1.png\" width=\"1540\" height=\"357\" srcset=\"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-content\/uploads\/sites\/4\/2024\/10\/PI-1.png 1540w, https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-content\/uploads\/sites\/4\/2024\/10\/PI-1-300x70.png 300w, https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-content\/uploads\/sites\/4\/2024\/10\/PI-1-1024x237.png 1024w, https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-content\/uploads\/sites\/4\/2024\/10\/PI-1-768x178.png 768w, https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-content\/uploads\/sites\/4\/2024\/10\/PI-1-1536x356.png 1536w\" sizes=\"(max-width: 1540px) 100vw, 1540px\" title=\"PI 1\" alt=\"\" \t\tclass=\"so-widget-image\"\/>\n\t<\/div>\n\n<\/div><\/div><div id=\"panel-319-0-0-2\" class=\"so-panel widget widget_sow-editor\" data-index=\"2\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<p style=\"text-align: center;\"><em>Stopped-flow coupled with absorption\/fluorescence system and \u00c4kta chromatography system.<\/em><\/p>\n<\/div>\n<\/div><\/div><div id=\"panel-319-0-0-3\" class=\"so-panel widget widget_sow-editor\" data-index=\"3\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>Chromatography Systems: \u00c4kta system equipped with columns for affinity, ion-exchange, and size-exclusion chromatography for protein purification, HPLC, FPLC.<\/li>\n<li>Cell Disruption: Sonicators and a cell disruptor (Constant Systems).<\/li>\n<li>Electrophoresis, Gel Reader: Fluorescence\/BET gel reader, PCR, western blot, rotary evaporator.<\/li>\n<li>Centrifuges: For various volumes (\u00b5L to L), including a Beckman Optima XE90 ultracentrifuge (maximum speed 90,000 rpm, 0-40\u00b0C) and an AirFuge.<\/li>\n<li>-80\u00b0C Freezers.<\/li>\n<li>Biosafety Cabinets (MSC) and Refrigerated Shaker Incubators.<\/li>\n<li>L2-Type Laboratory.<\/li>\n<li>Electroporation and Nucleofection Systems: 4DNucleofector, Lonza.<\/li>\n<li>Sterilization and Biological Waste Disposal Systems: Autoclaves.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div><\/div><div id=\"panel-319-0-0-4\" class=\"so-panel widget widget_sow-image\" data-index=\"4\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-image so-widget-sow-image-default-c67d20f9f743-319\"\n\t\t\t\n\t\t>\n<div class=\"sow-image-container\">\n\t\t<img \n\tsrc=\"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-content\/uploads\/sites\/4\/2024\/10\/P2.jpg\" width=\"1390\" height=\"660\" srcset=\"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-content\/uploads\/sites\/4\/2024\/10\/P2.jpg 1390w, https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-content\/uploads\/sites\/4\/2024\/10\/P2-300x142.jpg 300w, https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-content\/uploads\/sites\/4\/2024\/10\/P2-1024x486.jpg 1024w, https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-content\/uploads\/sites\/4\/2024\/10\/P2-768x365.jpg 768w\" sizes=\"(max-width: 1390px) 100vw, 1390px\" alt=\"\" \t\tclass=\"so-widget-image\"\/>\n\t<\/div>\n\n<\/div><\/div><div id=\"panel-319-0-0-5\" class=\"so-panel widget widget_sow-editor\" data-index=\"5\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<p style=\"text-align: center;\"><em>Overview of the L2-type laboratory (cell line culture and handling).<\/em><\/p>\n<\/div>\n<\/div><\/div><div id=\"panel-319-0-0-6\" class=\"so-panel widget widget_sow-editor\" data-index=\"6\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>Spectrophotometry: UV-Vis spectrophotometers (single and double beam), IR and fluorescence spectrometers (Horiba), and a stopped-flow apparatus with absorption and fluorescence detection (Applied Photophysics).<\/li>\n<li>Dynamic Light Scattering (DLS): For measuring nanoparticle size and Zeta potential (Zetasizer, Malvern).<\/li>\n<li>Isothermal Titration Calorimetry (Nano ITC, TA Instruments).<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div><\/div><div id=\"panel-319-0-0-7\" class=\"so-panel widget widget_sow-editor panel-last-child\" data-index=\"7\" ><div class=\"panel-widget-style panel-widget-style-for-319-0-0-7\" ><div\n\t\t\t\n\t\t\tclass=\"so-widget-sow-editor so-widget-sow-editor-base\"\n\t\t\t\n\t\t><h3 class=\"widget-title\">Contact<\/h3>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<p><a href=\"https:\/\/www.icp.universite-paris-saclay.fr\/contact-on\/\" target=\"_blank\" rel=\"noopener\">Oliver N\u00fcsse<\/a><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>The CPSysBio group has an extensive range of instruments that support not only biochemical, molecular, and cellular biology experiments but also the detailed characterization of nanoparticles. Accessible to group members and the ICP, the equipment is also available to external users under specific conditions. Stopped-flow coupled with absorption\/fluorescence system and \u00c4kta chromatography system. Chromatography Systems:&hellip;&nbsp;<a href=\"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/en\/technical-equipment\/\" rel=\"bookmark\">Lire la suite &raquo;<span class=\"screen-reader-text\">Technical Equipment<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"off","neve_meta_content_width":100,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","footnotes":""},"class_list":["post-319","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/pages\/319","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/comments?post=319"}],"version-history":[{"count":2,"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/pages\/319\/revisions"}],"predecessor-version":[{"id":403,"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/pages\/319\/revisions\/403"}],"wp:attachment":[{"href":"https:\/\/www.icp.universite-paris-saclay.fr\/cpsysbio\/wp-json\/wp\/v2\/media?parent=319"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}