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FT-ICR Mass Spectrometer at Very High Resolution

FT-ICR Mass Spectrometer at Very High Resolution -- [E10]

Infrared Spectroscopy of Ions Integrated with Mass Spectrometry / 7T FT-ICR

The room is equipped with a hybrid FT-ICR mass spectrometer featuring a 7T magnet (Bruker Apex Qe), coupled with the Free Electron Laser, an OPO/OPA tabletop laser (2.5 -5 µm), and a CO2 laser (10.6 µm). This setup allows for deriving IR spectra of ions within the mass spectrometer. Ions are vaporized using an ESI or nanoESI source. The Ar introduction line used for CID (Collision-Induced Dissociation) fragmentation has been modified to introduce neutral molecules directly into the collision cell, enabling ion-molecule reactions. This device is part of the national INFRANALYTICS network and PEPR LUMA.

Disciplines

                • Molecular Physicochemistry
                • Analytical Chemistry

Complementary Techniques

                • High-resolution mass spectrometry (100,000)
                • Tandem mass spectrometry (MS2) and MSn
                • ECD
                • Nanospray

Applications

                • Two-color IR Spectroscopy
                • Characterization of ions produced by ECD (Electron Capture Dissociation)
                • Study of reaction mechanisms via spectroscopic intermediates
                • Characterization of products in complex mixtures
                • Characterization of oxidation and fragmentation products of peptides
                • Study of ion-molecule reactions

Sample Environment

                • Electrospray and nanospray sources
                • Solution concentration ~ 10⁻⁵ to 10⁻⁹ M, typically in a water: methanol mixture
                • Ability to introduce solid, liquid, or gaseous neutral samples into the hexapod used as a collision cell.

Energy Range

Infrared :

                • 5 to 20 µm (CLIO)
                • 2.5 to 5 µm (OPO/OPA)

Detectors / Complementary Equipment

                • Infrared-sensitive detectors
                • OPO/OPA tabletop laser
                • CO₂ laser

Beam Size

Adjustable.

The infrared beam is focused within the ICR cell through a hole about 6 mm in diameter.

Technical Details

                • FT-ICR Mass Spectrometer: Equipped with electrospray and nanospray sources. The sample solution is introduced into the spectrometer using a syringe pump with a constant flow rate of about 120 µL/h. Pressure is approximately 10⁻¹⁰ Torr. Resolution is around 1,000,000 at m/z 4000.
                • OPO/OPA Laser (Laser Vision): Includes a Nd pump laser, an OPO, and an OPA. KTP (KTiOPO₄) and KTA (KTiOAsO₄) crystals are used to sequentially change the wavelength to the desired value.
                • Chemistry Laboratory: Used for sample preparation, equipped with a balance, ultrasonic bath, and Sorbonne.

Contact

Head of Experiments : Debora Scuderi