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Ultraviolet Photodissociation and Hydrogen/Deuterium Exchange for Protein Structure and Oxidation – UPHEBOS Project

Guillaume van der Rest (Prof), Isabelle Billault (Associate Prof), Eva Audouin (PhD)

The UPHEBOS project focuses on the analysis of protein conformations and structures. It aims to develop a comprehensive UVPD/MS/MS methodology utilizing hydrogen/deuterium exchange, applied to the structural characterization of proteins involved in oxidative stress phenomena. A physicochemical approach is underway, involving the development of an optimized experimental setup for low-temperature chromatography and online digestion. This will enable detailed analysis of the kinetics and mechanisms involved in hydrogen/deuterium exchange. Ultimately, the methodology will be applied to study oxidative modifications in proteins.